Sense-, antisense- and RNAi-4CL1 regulate soluble phenolic acids, cell wall components and growth in transgenic Populus tomentosa Carr.
Identifieur interne : 002493 ( Main/Exploration ); précédent : 002492; suivant : 002494Sense-, antisense- and RNAi-4CL1 regulate soluble phenolic acids, cell wall components and growth in transgenic Populus tomentosa Carr.
Auteurs : Xiaoming Tian [République populaire de Chine] ; Jin Xie ; Yanling Zhao ; Hai Lu ; Shichang Liu ; Long Qu ; Jianmei Li ; Ying Gai ; Xiangning JiangSource :
- Plant physiology and biochemistry : PPB [ 1873-2690 ] ; 2013.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Populus, Protéines végétales, Végétaux génétiquement modifiés.
- métabolisme : Hydroxybenzoates, Lignine, Paroi cellulaire, Populus, Protéines végétales, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Hydroxybenzoates, Lignin, Plant Proteins.
- genetics : Plants, Genetically Modified, Populus.
- metabolism : Cell Wall, Plants, Genetically Modified, Populus.
Abstract
Regulation of lignin biosynthesis affects plant growth and wood properties. Transgenic downregulation of 4-coumarate:coenzyme A ligase (4CL, EC 6.2.1.12) may reduce lignin content in cell walls, which could improve the qualities of pulp in papermaking and increase the efficiency of bioenergy applications. To determine the effects of Ptc4CL1 on lignin biosynthesis and plant growth, Populus tomentosa Carr. was transformed using sense-, antisense-, and RNAi-4CL1 genes. The growth properties, gene expression, enzyme activity, lignin content and composition and content of soluble phenolic acids were investigated in 1-year-old field-grown transgenic poplar trees. Transgenic up- and down-regulation of 4CL1 altered lignin content and composition in transgenic poplars, but there were no negative effects on the growth of transgenic plants. In addition, the severe changes in auxin observed in transgenic lines led to significantly enhanced growth performance. Furthermore, lignin content was tightly correlated with the alteration of 4CL1 enzymatic activity, which was correlated with 4CL1 gene expression. A significant increase in S units in lignin with a slight increase in sinapic acid was observed in 4CL1 down-regulated transgenic poplars. These results suggest that 4CL1 is a traffic control gene in monolignol biosynthesis and confirm that 4CL1 activity has been implicated with sinapoyl activation. Finally, our data demonstrate that there is cross-correlation among 4CL1 gene expression, 4CL1 enzyme activity, soluble phenolic acid, lignin monomer biosynthesis, and lignin content.
DOI: 10.1016/j.plaphy.2013.01.010
PubMed: 23434928
Affiliations:
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last="Zhao">Yanling Zhao</name></author><author><name sortKey="Lu, Hai" sort="Lu, Hai" uniqKey="Lu H" first="Hai" last="Lu">Hai Lu</name></author><author><name sortKey="Liu, Shichang" sort="Liu, Shichang" uniqKey="Liu S" first="Shichang" last="Liu">Shichang Liu</name></author><author><name sortKey="Qu, Long" sort="Qu, Long" uniqKey="Qu L" first="Long" last="Qu">Long Qu</name></author><author><name sortKey="Li, Jianmei" sort="Li, Jianmei" uniqKey="Li J" first="Jianmei" last="Li">Jianmei Li</name></author><author><name sortKey="Gai, Ying" sort="Gai, Ying" uniqKey="Gai Y" first="Ying" last="Gai">Ying Gai</name></author><author><name sortKey="Jiang, Xiangning" sort="Jiang, Xiangning" uniqKey="Jiang X" first="Xiangning" last="Jiang">Xiangning Jiang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23434928</idno><idno type="pmid">23434928</idno><idno type="doi">10.1016/j.plaphy.2013.01.010</idno><idno 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Forestry University, Beijing 100083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Xie, Jin" sort="Xie, Jin" uniqKey="Xie J" first="Jin" last="Xie">Jin Xie</name></author><author><name sortKey="Zhao, Yanling" sort="Zhao, Yanling" uniqKey="Zhao Y" first="Yanling" last="Zhao">Yanling Zhao</name></author><author><name sortKey="Lu, Hai" sort="Lu, Hai" uniqKey="Lu H" first="Hai" last="Lu">Hai Lu</name></author><author><name sortKey="Liu, Shichang" sort="Liu, Shichang" uniqKey="Liu S" first="Shichang" last="Liu">Shichang Liu</name></author><author><name sortKey="Qu, Long" sort="Qu, Long" uniqKey="Qu L" first="Long" last="Qu">Long Qu</name></author><author><name sortKey="Li, Jianmei" sort="Li, Jianmei" uniqKey="Li J" first="Jianmei" last="Li">Jianmei Li</name></author><author><name sortKey="Gai, Ying" sort="Gai, Ying" uniqKey="Gai Y" first="Ying" last="Gai">Ying Gai</name></author><author><name sortKey="Jiang, Xiangning" sort="Jiang, Xiangning" uniqKey="Jiang X" first="Xiangning" last="Jiang">Xiangning Jiang</name></author></analytic><series><title level="j">Plant physiology and biochemistry : PPB</title><idno type="eISSN">1873-2690</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (metabolism)</term><term>Hydroxybenzoates (metabolism)</term><term>Lignin (metabolism)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Hydroxybenzoates (métabolisme)</term><term>Lignine (métabolisme)</term><term>Paroi cellulaire (métabolisme)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Hydroxybenzoates</term><term>Lignin</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term><term>Protéines végétales</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Hydroxybenzoates</term><term>Lignine</term><term>Paroi cellulaire</term><term>Populus</term><term>Protéines végétales</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Regulation of lignin biosynthesis affects plant growth and wood properties. Transgenic downregulation of 4-coumarate:coenzyme A ligase (4CL, EC 6.2.1.12) may reduce lignin content in cell walls, which could improve the qualities of pulp in papermaking and increase the efficiency of bioenergy applications. To determine the effects of Ptc4CL1 on lignin biosynthesis and plant growth, Populus tomentosa Carr. was transformed using sense-, antisense-, and RNAi-4CL1 genes. The growth properties, gene expression, enzyme activity, lignin content and composition and content of soluble phenolic acids were investigated in 1-year-old field-grown transgenic poplar trees. Transgenic up- and down-regulation of 4CL1 altered lignin content and composition in transgenic poplars, but there were no negative effects on the growth of transgenic plants. In addition, the severe changes in auxin observed in transgenic lines led to significantly enhanced growth performance. Furthermore, lignin content was tightly correlated with the alteration of 4CL1 enzymatic activity, which was correlated with 4CL1 gene expression. A significant increase in S units in lignin with a slight increase in sinapic acid was observed in 4CL1 down-regulated transgenic poplars. These results suggest that 4CL1 is a traffic control gene in monolignol biosynthesis and confirm that 4CL1 activity has been implicated with sinapoyl activation. Finally, our data demonstrate that there is cross-correlation among 4CL1 gene expression, 4CL1 enzyme activity, soluble phenolic acid, lignin monomer biosynthesis, and lignin content.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23434928</PMID><DateCompleted><Year>2013</Year><Month>09</Month><Day>05</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2690</ISSN><JournalIssue CitedMedium="Internet"><Volume>65</Volume><PubDate><Year>2013</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Plant physiology and biochemistry : PPB</Title><ISOAbbreviation>Plant Physiol Biochem</ISOAbbreviation></Journal><ArticleTitle>Sense-, antisense- and RNAi-4CL1 regulate soluble phenolic acids, cell wall components and growth in transgenic Populus tomentosa Carr.</ArticleTitle><Pagination><MedlinePgn>111-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.plaphy.2013.01.010</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0981-9428(13)00029-6</ELocationID><Abstract><AbstractText>Regulation of lignin biosynthesis affects plant growth and wood properties. Transgenic downregulation of 4-coumarate:coenzyme A ligase (4CL, EC 6.2.1.12) may reduce lignin content in cell walls, which could improve the qualities of pulp in papermaking and increase the efficiency of bioenergy applications. To determine the effects of Ptc4CL1 on lignin biosynthesis and plant growth, Populus tomentosa Carr. was transformed using sense-, antisense-, and RNAi-4CL1 genes. The growth properties, gene expression, enzyme activity, lignin content and composition and content of soluble phenolic acids were investigated in 1-year-old field-grown transgenic poplar trees. Transgenic up- and down-regulation of 4CL1 altered lignin content and composition in transgenic poplars, but there were no negative effects on the growth of transgenic plants. In addition, the severe changes in auxin observed in transgenic lines led to significantly enhanced growth performance. Furthermore, lignin content was tightly correlated with the alteration of 4CL1 enzymatic activity, which was correlated with 4CL1 gene expression. A significant increase in S units in lignin with a slight increase in sinapic acid was observed in 4CL1 down-regulated transgenic poplars. These results suggest that 4CL1 is a traffic control gene in monolignol biosynthesis and confirm that 4CL1 activity has been implicated with sinapoyl activation. Finally, our data demonstrate that there is cross-correlation among 4CL1 gene expression, 4CL1 enzyme activity, soluble phenolic acid, lignin monomer biosynthesis, and lignin content.</AbstractText><CopyrightInformation>Copyright © 2013 Elsevier Masson SAS. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tian</LastName><ForeName>Xiaoming</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Life Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, PR China. tianxiaoming1986@126.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xie</LastName><ForeName>Jin</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Yanling</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Hai</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Shichang</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Qu</LastName><ForeName>Long</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jianmei</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Gai</LastName><ForeName>Ying</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Xiangning</ForeName><Initials>X</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>01</Month><Day>31</Day></ArticleDate></Article><MedlineJournalInfo><Country>France</Country><MedlineTA>Plant Physiol Biochem</MedlineTA><NlmUniqueID>9882449</NlmUniqueID><ISSNLinking>0981-9428</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D062385">Hydroxybenzoates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>I3P9R8317T</RegistryNumber><NameOfSubstance UI="C017616">phenolic acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D062385" MajorTopicYN="N">Hydroxybenzoates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" 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PubStatus="entrez"><Year>2013</Year><Month>2</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>2</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>9</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23434928</ArticleId><ArticleId IdType="pii">S0981-9428(13)00029-6</ArticleId><ArticleId IdType="doi">10.1016/j.plaphy.2013.01.010</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Gai, Ying" sort="Gai, Ying" uniqKey="Gai Y" first="Ying" last="Gai">Ying Gai</name><name sortKey="Jiang, Xiangning" sort="Jiang, Xiangning" uniqKey="Jiang X" first="Xiangning" last="Jiang">Xiangning Jiang</name><name sortKey="Li, Jianmei" sort="Li, Jianmei" uniqKey="Li J" first="Jianmei" last="Li">Jianmei Li</name><name sortKey="Liu, Shichang" sort="Liu, Shichang" uniqKey="Liu S" first="Shichang" last="Liu">Shichang Liu</name><name sortKey="Lu, Hai" sort="Lu, Hai" uniqKey="Lu H" first="Hai" last="Lu">Hai Lu</name><name sortKey="Qu, Long" sort="Qu, Long" uniqKey="Qu L" first="Long" last="Qu">Long Qu</name><name sortKey="Xie, Jin" sort="Xie, Jin" uniqKey="Xie J" first="Jin" last="Xie">Jin Xie</name><name sortKey="Zhao, Yanling" sort="Zhao, Yanling" uniqKey="Zhao Y" first="Yanling" last="Zhao">Yanling Zhao</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Tian, Xiaoming" sort="Tian, Xiaoming" uniqKey="Tian X" first="Xiaoming" last="Tian">Xiaoming Tian</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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